From Worms to Flies and Mice: Novel approaches for In-vivo Optical Tomography

28 Août 2012

Séminaire

There is growing interest in imaging dynamic systems such as a developing organism, and lately imaging techniques have shifted from imaging the very small to imaging larger samples and specimens in-toto. However, the use of these techniques is hampered by the presence of scattering, a seemingly all-present enemy. In this talk I will present new advances towards obtaining 3D fluorescence images in live specimens and how instead of bypassing the effect of scattering we can proceed differently by understanding this process and including it in our light propagation model. To this effect, different techniques will be presented that deal with different amounts of scattering from the transparent samples (such as Selective Plane Illumination Microscopy), to slightly scattering samples (such as Optical Projection Tomography), up to highly scattering media (such as Fluorescence Molecular Tomography). The basics of scattering and light propagation will be explained with practical examples and the different techniques which will be categorized depending on the amount of scattering present. Examples presented here include fluorescence imaging of live animals such as C. elegans, D. melanogaster development and tumor imaging in mice.

Intervenants / conférenciers:
Jorge Ripoll

Organisation:
LiMat
Email: aline.herren@unifr.ch    T: 0263009253    

Date et heure: 28 Août 2012 10:15

Lieu: Pérolles, Auditorium 0.51 (Chemin du Musée 3, Fribourg)

Rubrique: Physique,Physik

  abonnement RSS        annoncer un événement       tout le memento